MAG/Siglec-4a Antibody (PSH02-42)

Western Blot: MAG/Siglec-4a Antibody (PSH02-42) [NBP3-32558]

Western Blot: MAG/Siglec-4a Antibody (PSH02-42) [NBP3-32558] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32558) at 1/2,000 dilution.

Lane 1: Mouse brain(no heat) tissue lysate
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Mouse liver tissue lysate (negative)
Lane 4: Rat brain(no heat) tissue lysate
Lane 5: Rat cerebellum tissue lysate
Lane 6: Rat liver tissue lysate (negative)

Lysates/proteins at 20 ug/Lane.

Predicted band size: 69 kDa
Observed band size: 100 kDa

Exposure time: 42 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32558) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-42) [NBP3-32558]

Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-42) [NBP3-32558] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32558) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32558) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-42) [NBP3-32558]

Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-42) [NBP3-32558] - Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling MAG/Siglec-4a with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32558) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32558, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).