MAG/Siglec-4a Antibody (refanezumab) - Humanized

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28053

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # refanezumab

Concentration

LYOPH mg/ml

View all MAG/Siglec-4a Antibodies »

Product Specifications

Immunogen

Siglec-4a / MAG

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Also known as 'refanezumab'.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MAG/Siglec-4a Antibody (refanezumab) - Humanized

MAG/Siglec-4a Antibody (refanezumab) - Humanized

ELISA: MAG/Siglec-4a Antibody (refanezumab) - Humanized[NBP3-28053] -

Immobilized human Siglec 4a / MAG, Fc Tag at 2 ug/mL can bind MAG/Siglec-4a Antibody (refanezumab) - Humanized, EC50=0.007489 ug/mL.

Applications for MAG/Siglec-4a Antibody (refanezumab) - Humanized

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MAG/Siglec-4a

Myelin-associated glycoprotein (MAG), a membrane glycoprotein of 100 kDa, is thought to be involved in the process of myelination (1). Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG (2). MAG has been implicated in inhibition of nerve regeneration in the CNS. This results from interactions between MAG and the Nogo receptor and gangliosides on the apposing axon, which generates intracellular inhibitory signals in the neuron (3). Results also suggest that MAG binds to a specific receptor and initiates a signal transduction cascade to effect inhibition. These results indicate that soluble dMAG detected in vivo could contribute to the lack of regeneration in the mammalian CNS after injury (4).

Long Name

Myelin-associated Glycoprotein

Alternate Names

Siglec-4a, Siglec4a

Gene Symbol

MAG

UniProt

P20916

Additional MAG/Siglec-4a Products

Product Documents for MAG/Siglec-4a Antibody (refanezumab) - Humanized

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MAG/Siglec-4a Antibody (refanezumab) - Humanized

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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