MAGEA1 Antibody (PD00-50)

Western Blot: MAGEA1 Antibody (PD00-50) [NBP3-32559]

Western Blot: MAGEA1 Antibody (PD00-50) [NBP3-32559] - Western blot analysis of MAGEA1 on A431 cell lysates with Rabbit anti-MAGEA1 antibody (NBP3-32559) at 1/1,000 dilution.

Lysates/proteins at 20 ug/Lane.

Predicted band size: 34 kDa
Observed band size: 45 kDa

Exposure time: 25 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32559) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: MAGEA1 Antibody (PD00-50) [NBP3-32559]

Immunohistochemistry: MAGEA1 Antibody (PD00-50) [NBP3-32559] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-MAGEA1 antibody (NBP3-32559) at 1/1,500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32559) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.