MAGEA10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06272

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MAGEA10 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human MAGEA10 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MAGEA10 Antibody

Western Blot: MAGEA10 Antibody [NBP3-06272]

Western Blot: MAGEA10 Antibody [NBP3-06272]

Western Blot: MAGEA10 Antibody [NBP3-06272] - Anti-MAGEA10 rabbit polyclonal antibody at 1:500 dilutionLane A: HL60 Whole Cell LysateLane B: U-251 MGWhole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:43 kDa(We are unsure as to the identity of these extra bands.)
Immunohistochemistry-Paraffin: MAGEA10 Antibody [NBP3-06272]

Immunohistochemistry-Paraffin: MAGEA10 Antibody [NBP3-06272]

Immunohistochemistry-Paraffin: MAGEA10 Antibody [NBP3-06272] - Immunochemical staining of human MAGEA10 in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: MAGEA10 Antibody [NBP3-06272]

Immunoprecipitation: MAGEA10 Antibody [NBP3-06272]

Immunoprecipitation: MAGEA10 Antibody [NBP3-06272] - MAGEA10 was immunoprecipitated using:Lane A:0.5 mg U-251 MG Whole Cell Lysate4 uL anti-MAGEA10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-MAGEA10 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size :45 kDa

Applications for MAGEA10 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAGEA10

MAGEA10 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternate Names

Cancer/testis antigen 1.10, CT1.10member 10, MAGE-10 antigen, melanoma antigen family A, 10, melanoma-associated antigen 10

Gene Symbol

MAGEA10

Additional MAGEA10 Products

Product Documents for MAGEA10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAGEA10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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