MAGEA3 Antibody (6D10)
Novus Biologicals, part of Bio-Techne | Catalog # H00004102-M01
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Sandwich ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 6D10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MAGEA3 (NP_005353, 44 a.a. ~ 114 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVA
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28489076).
Specificity
MAGEA3 - melanoma antigen family A, 3
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for MAGEA3 Antibody (6D10)
![Western Blot: MAGEA3 Antibody (6D10) [H00004102-M01]](https://resources.bio-techne.com/images/products/MAGEA3-Antibody-6D10-Western-Blot-H00004102-M01-img0005.jpg "Western Blot: MAGEA3 Antibody (6D10) [H00004102-M01]")
Western Blot: MAGEA3 Antibody (6D10) [H00004102-M01]
Western Blot: MAGEA3 Antibody (6D10) [H00004102-M01] - Analysis of MAGEA3 expression in transfected 293T cell line by MAGEA3 monoclonal antibody (M01), clone 6D10.Lane 1: MAGEA3 transfected lysate (Predicted MW: 34.7 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: MAGEA3 Antibody (6D10) [H00004102-M01] - Detection limit for recombinant GST tagged MAGEA3 is approximately 0.3ng/ml as a capture antibody.
Applications for MAGEA3 Antibody (6D10)
Application
Recommended Usage
Sandwich ELISA
1:100-1:2000
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MAGEA3
Long Name
Melanoma-Associated Antigen 3
Alternate Names
CT1.3, MAGE3
Entrez Gene IDs
4102 (Human)
Gene Symbol
MAGEA3
OMIM
300174 (Human)
UniProt
Additional MAGEA3 Products
Product Documents for MAGEA3 Antibody (6D10)
Product Specific Notices for MAGEA3 Antibody (6D10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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