MAGEA4 Antibody (3D12)

Novus Biologicals, part of Bio-Techne | Catalog # H00004103-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3D12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAGEA4 Antibodies »

Product Specifications

Immunogen

MAGEA4 (NP_002353, 98 a.a. ~ 171 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVD

Specificity

melanoma antigen family A, 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MAGEA4 Antibody (3D12)

Western Blot: MAGEA4 Antibody (3D12) [H00004103-M01]

Western Blot: MAGEA4 Antibody (3D12) [H00004103-M01]

Western Blot: MAGEA4 Antibody (3D12) [H00004103-M01] - MAGEA4 monoclonal antibody (M01), clone 3D12 Analysis of MAGEA4 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: MAGEA4 Antibody (3D12) [H00004103-M01]

Immunocytochemistry/ Immunofluorescence: MAGEA4 Antibody (3D12) [H00004103-M01]

Immunocytochemistry/Immunofluorescence: MAGEA4 Antibody (3D12) [H00004103-M01] - Analysis of monoclonal antibody to MAGEA4 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: MAGEA4 Antibody (3D12) [H00004103-M01] - Detection limit for recombinant GST tagged MAGEA4 is approximately 0.3ng/ml as a capture antibody.

Applications for MAGEA4 Antibody (3D12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate for WB. It has been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAGEA4

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene.

Long Name

Melanoma-Associated Antigen 4

Alternate Names

CT1.4, MAGE-41, MAGE-X2, MAGE4A, MAGE4B

Entrez Gene IDs

4103 (Human)

Gene Symbol

MAGEA4

OMIM

300175 (Human)

UniProt

NP_002353

Additional MAGEA4 Products

Product Documents for MAGEA4 Antibody (3D12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAGEA4 Antibody (3D12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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