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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human MAGEA4(NP_001011550) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

34.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAGEA4 Antibody (OTI2C1) - Azide and BSA Free

Western Blot: MAGEA4 Antibody (OTI2C1)Azide and BSA Free [NBP2-72571]

Western Blot: MAGEA4 Antibody (OTI2C1)Azide and BSA Free [NBP2-72571]

Western Blot: MAGEA4 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72571] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA4.
Immunocytochemistry/ Immunofluorescence: MAGEA4 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72571]

Immunocytochemistry/ Immunofluorescence: MAGEA4 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72571]

Immunocytochemistry/Immunofluorescence: MAGEA4 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72571] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MAGEA4.

Applications for MAGEA4 Antibody (OTI2C1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAGEA4

MAGEA4 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene.

Long Name

Melanoma-Associated Antigen 4

Alternate Names

CT1.4, MAGE-41, MAGE-X2, MAGE4A, MAGE4B

Gene Symbol

MAGEA4

Additional MAGEA4 Products

Product Documents for MAGEA4 Antibody (OTI2C1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAGEA4 Antibody (OTI2C1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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