Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all MAK10 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human MAK10. The immunogen is located within amino acids 190 - 240 of MAK10. Amino Acid Squence: KDVEDDMQRRVKSTRSRQGE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAK10 Antibody - BSA Free

Western Blot: MAK10 AntibodyBSA Free [NBP1-77056]

Western Blot: MAK10 AntibodyBSA Free [NBP1-77056]

Western Blot: MAK10 Antibody [NBP1-77056] - Rat heart tissue lysate with MAK10 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking peptide.
Immunohistochemistry-Paraffin: MAK10 Antibody - BSA Free [NBP1-77056]

Immunohistochemistry-Paraffin: MAK10 Antibody - BSA Free [NBP1-77056]

Immunohistochemistry-Paraffin: MAK10 Antibody [NBP1-77056] - Human heart tissue with MAK10 antibody at 2.5 ug/ml.
MAK10 Antibody - BSA Free

Immunohistochemistry: MAK10 Antibody - BSA Free [NBP1-77056] -

Immunohistochemistry: MAK10 Antibody - BSA Free [NBP1-77056] - Immunohistochemistry of MAK10 in human heart tissue with MAK10 antibody at 2.5 u/mL.
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Applications for MAK10 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAK10

The MAK10 gene encodes a 733-amino acid protein with several regions of similarity to T cell receptor alpha-subunit V (variable) regions in yeast. The mammalian homologue of yeast MAK10, also known as EGAP, is one subunit of a novel N-terminal acetyltransferase (NAT) that is highly conserved among vertebrate species. It is expressed in a variety of tissues in the developing rat embryo but restricted in expression in the adult, remaining detectable only in tissues undergoing continual cell renewal or in cells responding to pathological injury. The MAK10-NAT complex is an essential regulatory enzyme controlling the function of a subset of proteins required for embryonic growth control and vessel development. This complex functionally co-assembles in mammalian cells to regulate cell proliferation and is essential for embryonic development, at least in part through the regulation of target of rapamycin (TOR) signaling events. At least two isoforms of MAK10 are known to exist.

Alternate Names

bA379P1.1, corneal wound healing-related protein, EGAP, embryonic growth-associated protein homolog, FLJ21613, FLJ22643, MAK10, MAK10 homolog, amino-acid N-acetyltransferase subunit, N(alpha)-acetyltransferase 35, NatC auxiliary subunit, N-alpha-acetyltransferase 35, NatC auxiliary subunit, protein MAK10 homolog

Gene Symbol

NAA35

UniProt

NP_078911 (Human)

Additional MAK10 Products

Product Documents for MAK10 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MAK10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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