Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MAML3 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human Mastermind-Like 3 using the numbering given in entry NP_061187.2 (GeneID 55534).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAML3 Antibody

Western Blot: MAML3 Antibody [NB100-2128]

Western Blot: MAML3 Antibody [NB100-2128]

Western Blot: MAML3 Antibody [NB100-2128] - Detection of Human and Mouse MAML3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody NB100-2128 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2128]

Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2128]

Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2128] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAML3 (NB100-2128). Detection: DAB
Immunoprecipitation: MAML3 Antibody [NB100-2128]

Immunoprecipitation: MAML3 Antibody [NB100-2128]

Immunoprecipitation: MAML3 Antibody [NB100-2128] - Detection of human MAML3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML3 antibody NB100-2128 used for IP at 6 ug per reaction. MAML3 was also immunoprecipitated by rabbit anti-MAML3 antibody NB100-2129. For blotting immunoprecipitated MAML3, NB100-2128 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for MAML3 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2- 10 ug/mg of lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MAML3

The three MAML genes are widely expressed in adult tissues but exhibit distinct expression patterns in mouse early spinal cord development. All MAML proteins localize to nuclear bodies, share a conserved basic domain in their N termini that binds to the ankyrin repeat domain of Notch, and contain a transcriptional activation domain in their C termini. MAML3 acts as a transcriptional coactivator for NOTCH proteins and has been shown to amplify NOTCH-induced transcription of HES1.

Alternate Names

CAG/CTG 3, ERDA3, MAM-2, Mam-3, mastermind-like 3 (Drosophila), mastermind-like protein 3, polyglutamine rich, trinucleotide repeat containing 3

Entrez Gene IDs

55534 (Human)

Gene Symbol

MAML3

UniProt

NP_061187.2 (Human)

Additional MAML3 Products

Product Documents for MAML3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAML3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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