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MAML3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2129

Novus Biologicals, part of Bio-Techne
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NB100-2129

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1133) human Mastermind-Like 3 using the numbering given in entry NP_061187.2 (GeneID 55534).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAML3 Antibody

Western Blot: MAML3 Antibody [NB100-2129]

Western Blot: MAML3 Antibody [NB100-2129]

Western Blot: MAML3 Antibody [NB100-2129] - Detection of Human and Mouse MAML3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody NB100-2129 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2129]

Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2129]

Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2129] - Detection of human MAML3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAML3 (NB100-2129). Detection: DAB
Immunoprecipitation: MAML3 Antibody [NB100-2129]

Immunoprecipitation: MAML3 Antibody [NB100-2129]

Immunoprecipitation: MAML3 Antibody [NB100-2129] - Detection of human MAML3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML3 antibody NB100-2129 (lot NB100-2129-2) used for IP at 6 ug per reaction. MAML3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2129-1) and rabbit anti-MAML3 antibody NB100-2128 For blotting immunoprecipitated MAML3, NB100-2129 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for MAML3 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200- 1:1000

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval withTris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MAML3

There are 3 MAML (mastermind-like) proteins in mammals (MAML1-3). MAML3 is a coactivator for NOTCH signaling. MAML3 associates with NOTCH and CSL in a ternary complex and functions to activate transcription of HES and hairy-related basic helix-loop-helix transcription factors.

Alternate Names

CAG/CTG 3, ERDA3, MAM-2, Mam-3, mastermind-like 3 (Drosophila), mastermind-like protein 3, polyglutamine rich, trinucleotide repeat containing 3

Entrez Gene IDs

55534 (Human); 433586 (Mouse)

Gene Symbol

MAML3

Additional MAML3 Products

Product Documents for MAML3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAML3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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