Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM518

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Mammaglobin A Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Mammaglobin A protein (Uniprot: Q13296)

Localization

Cytoplasmic, Cell Surface, Nuclear

Marker

Breast Cancer Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79729).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Mammaglobin A Antibody (SPM518) - Azide and BSA Free

Western Blot: Mammaglobin A Antibody (SPM518)Azide and BSA Free [NBP2-79864]

Western Blot: Mammaglobin A Antibody (SPM518)Azide and BSA Free [NBP2-79864]

Western Blot: Mammaglobin A Antibody (SPM518) - Azide and BSA Free [NBP2-79864] - Western Blot Analysis of Human MCF-7,T47D and MDA-231 cell lysate using Monospecific Mouse Monoclonal Antibody (SPM518) to Mammaglobin.
Immunohistochemistry-Paraffin: Mammaglobin A Antibody (SPM518) - Azide and BSA Free [NBP2-79864]

Immunohistochemistry-Paraffin: Mammaglobin A Antibody (SPM518) - Azide and BSA Free [NBP2-79864]

Immunohistochemistry-Paraffin: Mammaglobin A Antibody (SPM518) - Azide and BSA Free [NBP2-79864] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Monospecific Mouse Monoclonal Antibody (SPM518) to Mammaglobin.
Protein Array: Mammaglobin A Antibody (SPM518) - Azide and BSA Free [NBP2-79864]

Protein Array: Mammaglobin A Antibody (SPM518) - Azide and BSA Free [NBP2-79864]

Protein Array: Mammaglobin A Antibody (SPM518) - Azide and BSA Free [NBP2-79864] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Applications for Mammaglobin A Antibody (SPM518) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Mammaglobin A

Mammaglobin is a gene that is expressed almost exclusively in the normal breast epithelium and human breast cancer. It is a member of the secretoglobin gene family and forms a heterodimer with lipophilin B. It has been suggested that mammaglobin may be a useful marker for breast cancer clinical research. Studies investigating the detection of mRNA by RT PCR from circulating carcinoma cells in the peripheral blood of breast cancer patients have shown that mammaglobin is a highly specific marker and correlates with several prognostic factors, such as lymph node involvement.

Alternate Names

mammaglobin 1, mammaglobin A, Mammaglobin-1, MGB1mammaglobin-A, MGC71974, Secretoglobin family 2A member 2, secretoglobin, family 2A, member 2, UGB2mammaglobin-1

Gene Symbol

SCGB2A2

Additional Mammaglobin A Products

Product Documents for Mammaglobin A Antibody (SPM518) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Mammaglobin A Antibody (SPM518) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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