MANEA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82279

Novus Biologicals, part of Bio-Techne
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NBP1-82279
NBP1-82279-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MANEA Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LRPNTATFGAPFGLDLLPELHQRTIHLGKNFDFQKSDRINSETNTKNLKSVEITMKPSKASELNLDELPPLNNYLHVFYYSWYGNPQFDGKYIHWNHPVLEHWDPRIAKNYPQGRHNPPDDIGSSFYPELGSYSSRD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MANEA Antibody

Immunocytochemistry/ Immunofluorescence: MANEA Antibody [NBP1-82279]

Immunocytochemistry/ Immunofluorescence: MANEA Antibody [NBP1-82279]

Immunocytochemistry/Immunofluorescence: MANEA Antibody [NBP1-82279] - Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-82279]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-82279]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-82279] - Staining of human testis.
Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-82279]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-82279]

Immunohistochemistry-Paraffin: MANEA Antibody [NBP1-82279] - Staining of human lymph node shows moderate positivity in non-germinal center cells.
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Applications for MANEA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MANEA

N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM]

Alternate Names

alpha 1,2-endomannosidase, DKFZp686D20120, EC 3.2.1, EC 3.2.1.130, ENDO, Endo-alpha mannosidase, Endomannosidase, FLJ12838, glycoprotein endo-alpha-1,2-mannosidase, hEndo, Mandaselin, mannosidase, endo-alpha

Gene Symbol

MANEA

Additional MANEA Products

Product Documents for MANEA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MANEA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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