MAO-A Antibody (5D1-R)

Western Blot: MAO-A Antibody (5D1-R) [NBP3-32566]

Western Blot: MAO-A Antibody (5D1-R) [NBP3-32566] - Western blot analysis of MAO-A on different lysates with Mouse anti-MAO-A antibody (NBP3-32566) at 1/1,000 dilution.

Lane 1: Rat brain tissue lysate
Lane 2: Mouse brain tissue lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 60 kDa
Observed band size: 70 kDa

Exposure time: 25 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32566) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: MAO-A Antibody (5D1-R) [NBP3-32566]

Immunohistochemistry: MAO-A Antibody (5D1-R) [NBP3-32566] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MAO-A antibody (NBP3-32566) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32566) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.