MAP4K3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05154

Novus Biologicals, part of Bio-Techne
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NBP3-05154-100ul
NBP3-05154-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MAP4K3 Antibodies »

Product Summary for MAP4K3 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 755-894 of human MAP4K3 (NP_003609.2). ETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP4K3 Antibody - BSA Free

Western Blot: MAP4K3 AntibodyBSA Free [NBP3-05154]

Western Blot: MAP4K3 AntibodyBSA Free [NBP3-05154]

Western Blot: MAP4K3 Antibody [NBP3-05154] - Analysis of extracts of various cell lines, using MAP4K3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

Applications for MAP4K3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAP4K3

Several mammalian kinases have been identified which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include GLK, KHS, NIK, YSK1, HPK1, Krs-1, Krs-2 and human GC kinase. GLK (for GC-like kinase) is an 885 amino acid protein that shares a high degree of homology with GC kinase and HPK1. Like many of the STE20-like kinases, GLK specifically activates the JNK pathway. Epistasis studies with dominant negative mutants of MEKK1 suggest that GLK functions upstream of MEKK1 in the JNK signaling pathway.

Alternate Names

EC 2.7.11, EC 2.7.11.1, germinal center kinase-like kinase, Germinal center kinase-related protein kinase, GLKMEKKK 3, MAPK/ERK kinase kinase kinase 3, MAPKKKK3, MEK kinase kinase 3, mitogen-activated protein kinase kinase kinase kinase 3, RAB8IPL1

Gene Symbol

MAP4K3

Additional MAP4K3 Products

Product Documents for MAP4K3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MAP4K3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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