MAP4K5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83385

Novus Biologicals, part of Bio-Techne
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NBP1-83385
NBP1-83385-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAP4K5 Antibodies »

Product Summary for MAP4K5 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YPEDNFPDEEKASTIKHCPDSESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLPRKKDKRDFPKPA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP4K5 Antibody

Western Blot: MAP4K5 Antibody [NBP1-83385]

Western Blot: MAP4K5 Antibody [NBP1-83385]

Western Blot: MAP4K5 Antibody [NBP1-83385] - Analysis in human cell line A-431.
Immunocytochemistry/ Immunofluorescence: MAP4K5 Antibody [NBP1-83385]

Immunocytochemistry/ Immunofluorescence: MAP4K5 Antibody [NBP1-83385]

Immunocytochemistry/Immunofluorescence: MAP4K5 Antibody [NBP1-83385] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAP4K5 Antibody [NBP1-83385]

Immunohistochemistry-Paraffin: MAP4K5 Antibody [NBP1-83385]

Immunohistochemistry-Paraffin: MAP4K5 Antibody [NBP1-83385] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Applications for MAP4K5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP4K5

MAP4K5 encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Long Name

Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase

Alternate Names

GCKR, KHS1, MAPKKKK5

Gene Symbol

MAP4K5

Additional MAP4K5 Products

Product Documents for MAP4K5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAP4K5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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