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MAP4K6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32766

Novus Biologicals, part of Bio-Techne
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NBP1-32766

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for MAP4K6 Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MAP4K6. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (81%), Xenopus laevis (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAP4K6 Antibody

Western Blot: MAP4K6 Antibody [NBP1-32766]

Western Blot: MAP4K6 Antibody [NBP1-32766]

Western Blot: MAP4K6 Antibody [NBP1-32766] - Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: MAP4K6 Antibody [NBP1-32766]

Immunocytochemistry/ Immunofluorescence: MAP4K6 Antibody [NBP1-32766]

Immunocytochemistry/Immunofluorescence: MAP4K6 Antibody [NBP1-32766] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MAP4K6 protein stained by MAP4K6 antibody [C2C3-2], C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: MAP4K6 Antibody [NBP1-32766]

Immunohistochemistry-Paraffin: MAP4K6 Antibody [NBP1-32766]

Immunohistochemistry-Paraffin: MAP4K6 Antibody [NBP1-32766] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.

Applications for MAP4K6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAP4K6

MINK is a MSN type protein kinase. The protein has a structure similar to other germinal center kinases, and it has been shown to activate the c-Jun N-terminal kinase and p38 pathways.

Alternate Names

B55, EC 2.7.11, GCK family kinase MiNK, hMINK, hMINKbeta, MAP4K6EC 2.7.11.1, MAPK/ERK kinase kinase kinase 6, MEK kinase kinase 6, MGC21111, MINKMEKKK 6, Misshapen/NIK-related kinase, misshapen-like kinase 1, misshapen-like kinase 1 (zebrafish), Mitogen-activated protein kinase kinase kinase kinase 6, serine/threonine protein kinase, YSK2, ZC3

Gene Symbol

MINK1

Additional MAP4K6 Products

Product Documents for MAP4K6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAP4K6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.