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MARCH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59758

Novus Biologicals, part of Bio-Techne
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NBP1-59758

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to MARCH1(membrane-associated ring finger (C3HC4) 1) The peptide sequence was selected from the N terminal of 40238. Peptide sequence LQASSPTTGTAPRSQSRLSVCPSTQDICRICHCEGDEESPLITPCRCTGT. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for MARCH1 Antibody

Western Blot: MARCH1 Antibody [NBP1-59758]

Western Blot: MARCH1 Antibody [NBP1-59758]

Western Blot: MARCH1 Antibody [NBP1-59758] - Human Muscle lysate, concentration 0.2-1 ug/ml.
MARCH1 Antibody

Western Blot: MARCH1 Antibody [NBP1-59758] -

Western Blot: MARCH1 Antibody [NBP1-59758] - Western blot analysis and gel protein densitometry were used for quantification of MARCH1 (NBP1-59758) and Arginase 1 (NBP1-32731) proteins on transduced macrophage D: The gel image indicates the blot results 48 and 72 hr after transduction, compared with the non-transduced cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35656075/) licensed under a CC-BY license.

Applications for MARCH1 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MARCH1

MARCH1 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC, CD86 and FAS, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Alternate Names

E3 ubiquitin-protein ligase MARCH1, EC 6.3.2, EC 6.3.2.-, MARCH-IDKFZp564M1682, membrane-associated ring finger (C3HC4) 1, Membrane-associated RING finger protein 1, Membrane-associated RING-CH protein I, RING finger protein 171, RNF171FLJ20668

Gene Symbol

MARCH1

Additional MARCH1 Products

Product Documents for MARCH1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MARCH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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