MARCH10 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28483
Key Product Details
Species Reactivity
Validated:
Rat
Predicted:
Bovine (100%), Human (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-SRNYPQPRTEE corresponding to internal region according to NP_001094345.1, NP_689811.2.
Epitope
C terminus
Specificity
This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for MARCH10 Antibody
![Western Blot: MARCH10 Antibody [NBP1-28483]](https://resources.bio-techne.com/images/products/MARCH10-Antibody-Western-Blot-NBP1-28483-img0002.jpg "Western Blot: MARCH10 Antibody [NBP1-28483]")
Western Blot: MARCH10 Antibody [NBP1-28483]
Western Blot: MARCH10 Antibody [NBP1-28483] - (0.3ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: MARCH10 Antibody [NBP1-28483]](https://resources.bio-techne.com/images/products/MARCH10-Antibody-Immunohistochemistry-Paraffin-NBP1-28483-img0003.jpg "Immunohistochemistry-Paraffin: MARCH10 Antibody [NBP1-28483]")
Immunohistochemistry-Paraffin: MARCH10 Antibody [NBP1-28483]
Immunohistochemistry-Paraffin: MARCH10 Antibody [NBP1-28483] - (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for MARCH10 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:8000
Western Blot
1:100-1:2000
Application Notes
WB: Approx. 90 kDa band observed in rat testis lysates (calculated MW of 90.5 kDa band according to human NP_689811.2 and of 87.9 kDa band according to mouse NP_766156.2). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MARCH10
Alternate Names
EC 6.3.2.-, FLJ35757, MARCH-Xprobable E3 ubiquitin-protein ligase MARCH10, membrane-associated ring finger (C3HC4) 10, Membrane-associated RING finger protein 10, Membrane-associated RING-CH protein X, RING finger protein 190RNF190
Gene Symbol
MARCH10
UniProt
Additional MARCH10 Products
Product Documents for MARCH10 Antibody
Product Specific Notices for MARCH10 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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