MARCH10 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89833
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LPDRESATEKDRGGSENAKKSPLSWDTKSEPRQEVGVNAENVWSDCISVEHRPGTHDSEGYWKDYLNSSQNSLDYFISGRPISPRSSVNSSYNPPAS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MARCH10 Antibody
Immunocytochemistry/ Immunofluorescence: MARCH10 Antibody [NBP1-89833]
Immunocytochemistry/Immunofluorescence: MARCH10 Antibody [NBP1-89833] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.Immunohistochemistry-Paraffin: MARCH10 Antibody [NBP1-89833]
Immunohistochemistry-Paraffin: MARCH10 Antibody [NBP1-89833] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.Applications for MARCH10 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MARCH10
Alternate Names
EC 6.3.2.-, FLJ35757, MARCH-Xprobable E3 ubiquitin-protein ligase MARCH10, membrane-associated ring finger (C3HC4) 10, Membrane-associated RING finger protein 10, Membrane-associated RING-CH protein X, RING finger protein 190RNF190
Entrez Gene IDs
162333 (Human)
Gene Symbol
MARCH10
UniProt
Additional MARCH10 Products
Product Documents for MARCH10 Antibody
Product Specific Notices for MARCH10 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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