MARCH3 Antibody (1F6)
Novus Biologicals, part of Bio-Techne | Catalog # H00115123-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 1F6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MARCH3 (NP_848545, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTTSRCSHLPEVLPDCTSSAAPVVKTVEDCGSLVNGQPQYVMQVSAKDGQLLSTVVRTLATQSPFNDRPMCRICHEGSSQEDLLSPCECTGTLGTIHRSC
Specificity
Reacts with membrane-associated ring finger (C3HC4) 3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for MARCH3 Antibody (1F6)
![Western Blot: MARCH3 Antibody (1F6) [H00115123-M03]](https://resources.bio-techne.com/images/products/MARCH3-Antibody-1F6-Western-Blot-H00115123-M03-img0002.jpg "Western Blot: MARCH3 Antibody (1F6) [H00115123-M03]")
Western Blot: MARCH3 Antibody (1F6) [H00115123-M03]
Western Blot: MARCH3 Antibody (1F6) [H00115123-M03] - Analysis of MARCH3 expression in transfected 293T cell line by MARCH3 monoclonal antibody (M03), clone 1F6. Lane 1: MARCH3 transfected lysate (Predicted MW: 28.5 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: MARCH3 Antibody (1F6) [H00115123-M03] - Detection limit for recombinant GST tagged MARCH3 is 0.3 ng/ml as a capture antibody.
Applications for MARCH3 Antibody (1F6)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MARCH3
Alternate Names
EC 6.3.2, EC 6.3.2.-, MARCH-IIIFLJ20096, membrane-associated ring finger (C3HC4) 3, Membrane-associated RING finger protein 3, Membrane-associated RING-CH protein III, MGC48332, RING finger protein 173, RNF173E3 ubiquitin-protein ligase MARCH3
Entrez Gene IDs
115123 (Human)
Gene Symbol
MARCH3
UniProt
Additional MARCH3 Products
Product Documents for MARCH3 Antibody (1F6)
Product Specific Notices for MARCH3 Antibody (1F6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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