MARCH3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81260

Novus Biologicals, part of Bio-Techne
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NBP1-81260
NBP1-81260-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MARCH3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FRYHCRLYNEWRRTNQRVILLIPKSVNVPSNQPSLLGLHSVKRNSKETVV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MARCH3 Antibody

Western Blot: MARCH3 Antibody [NBP1-81260]

Western Blot: MARCH3 Antibody [NBP1-81260]

Western Blot: MARCH3 Antibody [NBP1-81260] - Analysis in control (vector only transfected HEK293T lysate) and LY405925 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MARCH3 Antibody [NBP1-81260]

Immunocytochemistry/ Immunofluorescence: MARCH3 Antibody [NBP1-81260]

Immunocytochemistry/Immunofluorescence: MARCH3 Antibody [NBP1-81260] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MARCH3 Antibody [NBP1-81260]

Immunohistochemistry-Paraffin: MARCH3 Antibody [NBP1-81260]

Immunohistochemistry-Paraffin: MARCH3 Antibody [NBP1-81260] - Staining of human kidney shows strong cytoplasmic positivity in a subset of renal tubules.

Applications for MARCH3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MARCH3

MARCH3 - membrane-associated ring finger (C3HC4) 3

Alternate Names

EC 6.3.2, EC 6.3.2.-, MARCH-IIIFLJ20096, membrane-associated ring finger (C3HC4) 3, Membrane-associated RING finger protein 3, Membrane-associated RING-CH protein III, MGC48332, RING finger protein 173, RNF173E3 ubiquitin-protein ligase MARCH3

Entrez Gene IDs

115123 (Human)

Gene Symbol

MARCH3

UniProt

Q86UD3 (Human)

Additional MARCH3 Products

Product Documents for MARCH3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MARCH3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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