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MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12048

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-12048-0.025mg
NBP3-12048-0.2mg

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # PLK1

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

The antibody was raised by immunising BALB/c mice with human alveolar macrophages (AMs) and subsequent creation of hybridomas.

Specificity

This antibody recognises the extracellular domain V between residues 420 and 431 in MARCO both in human and bovine samples.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free [NBP3-12048]

Immunocytochemistry/ Immunofluorescence: MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free [NBP3-12048]

Immunocytochemistry/Immunofluorescence: MARCO Antibody (PLK1) - Chimeric [NBP3-12048] - Immunofluorescence analysis of paraformaldehyde fixed Human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of PLK1 (NBP3-12048) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12048, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: MARCO

MARCO is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram negative and Gram positive bacteria via an extracellular, C terminal, scavenger receptor cysteine rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. MARCO may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains.

Long Name

Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2

Alternate Names

SCARA2

Gene Symbol

MARCO

Additional MARCO Products

Product Documents for MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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