Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3A5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MAST205 Antibodies »

Product Summary for MAST205 Antibody (OTI3A5) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 1482-1798 of human MAST2(NP_055927) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

196.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAST205 Antibody (OTI3A5) - Azide and BSA Free

Western Blot: MAST205 Antibody (OTI3A5)Azide and BSA Free [NBP2-72592]

Western Blot: MAST205 Antibody (OTI3A5)Azide and BSA Free [NBP2-72592]

Western Blot: MAST205 Antibody (OTI3A5) - Azide and BSA Free [NBP2-72592] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAST2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAST2.
Western Blot: MAST205 Antibody (OTI3A5)Azide and BSA Free [NBP2-72592]

Western Blot: MAST205 Antibody (OTI3A5)Azide and BSA Free [NBP2-72592]

Western Blot: MAST205 Antibody (OTI3A5) - Azide and BSA Free [NBP2-72592] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and MAST2-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-MAST2 monoclonal antibody. Then the blotted membrane was stripped and reprobed with

Applications for MAST205 Antibody (OTI3A5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAST205

MAST2 (microtubule-associated serine/threonine-protein kinase 2) is also known as MAST205. MAST2 contains a well conserved protein kinase C/A kinase domain and a PDZ domain. It is highly expressed in spermatids and has been shown to play a role in cytokine responses in macrophages.

Alternate Names

EC 2.7.11, EC 2.7.11.1, FLJ39200, KIAA0807RP4-533D7.1, MAST205microtubule associated testis specific serine/threonine protein kinase, microtubule associated serine/threonine kinase 2, microtubule-associated serine/threonine-protein kinase 2, MTSSK

Gene Symbol

MAST2

Additional MAST205 Products

Product Documents for MAST205 Antibody (OTI3A5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MAST205 Antibody (OTI3A5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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