MAST3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82993

Novus Biologicals, part of Bio-Techne
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NBP1-82993
NBP1-82993-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAST3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PVFILGEPDPPPAATPVMPKPSSLSADTAALSHARLRSNSIGARHSTPRPLDASRGRRLGGPRDPAPEKSR

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30449657). Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAST3 Antibody

Immunocytochemistry/ Immunofluorescence: MAST3 Antibody [NBP1-82993]

Immunocytochemistry/ Immunofluorescence: MAST3 Antibody [NBP1-82993]

Immunocytochemistry/Immunofluorescence: MAST3 Antibody [NBP1-82993] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAST3 Antibody [NBP1-82993]

Immunohistochemistry-Paraffin: MAST3 Antibody [NBP1-82993]

Immunohistochemistry-Paraffin: MAST3 Antibody [NBP1-82993] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.

Applications for MAST3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

Reported in scientific literature (PMID: 30449657)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAST3

Microtubule associated serine/threonine kinase 3 or MAST3 is involved with protein phosphorylation. Belonging to the AGC Ser/Thr protein kinase family, MAST3 are cytoskeletal kinases associated with ATP binding, magnesium ion binding, protein binding and protein serine/threonine kinase activity. Popular protein domains for MAST3 include PDZ, protein kinase, AGC-kinase C-terminal domain and domain of unknown function (DUF1908). MAST3 is also known to interact with PTEN, PRKAB2, PRKAA2, CNTNAP4 and YWHAH.

Alternate Names

EC 2.7.11, KIAA0561EC 2.7.11.1, microtubule associated serine/threonine kinase 3, microtubule-associated serine/threonine-protein kinase 3

Gene Symbol

MAST3

Additional MAST3 Products

Product Documents for MAST3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAST3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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