Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MASTL Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human microtubule associated serine/threonine kinase-like using the numbering given in entry NP_116233.2 (GeneID 84930).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MASTL Antibody

Western Blot: MASTL Antibody [NBP1-22987]

Western Blot: MASTL Antibody [NBP1-22987]

Western Blot: MASTL Antibody [NBP1-22987] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MASTL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-22987]

Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-22987]

Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-22987] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MASTL used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: MASTL Antibody [NBP1-22987]

Immunoprecipitation: MASTL Antibody [NBP1-22987]

Immunoprecipitation: MASTL Antibody [NBP1-22987] - Detection of human MASTL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MASTL antibody NBP1-22987 (lot NBP1-22987-4) used for IP at 3 ug per reaction. MASTL was also immunoprecipitated by a previous lot of this antibody (lot NBP1-22987-3). For blotting immunoprecipitated MASTL, NBP1-22987 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for MASTL Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MASTL

MASTL (microtubule-associated serine/threonine-protein kinase-like) is an uncharacterized putative serine/threonine kinase which may be involved in megakaryocyte development. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2) a disease characterized by decreased circulating platelets and increased potential for bleeding.

Long Name

MASTL

Alternate Names

Greatwall, THC2

Entrez Gene IDs

84930 (Human)

Gene Symbol

MASTL

UniProt

Q96GX5

Additional MASTL Products

Product Documents for MASTL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MASTL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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