MAT1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-55120

Novus Biologicals, part of Bio-Techne
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NBP1-55120
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bacteria

Cited:

Human, Mouse, Bacteria

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all MAT1A Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to MAT1A(methionine adenosyltransferase I, alpha) The peptide sequence was selected from the N terminal of MAT1A (NP_000420). Peptide sequence TSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGE. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Bacteria reactivity reported in scientific literature (PMID: 31363118).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for MAT1A Antibody

Western Blot: MAT1A Antibody [NBP1-55120]

Western Blot: MAT1A Antibody [NBP1-55120]

Western Blot: MAT1A Antibody [NBP1-55120] - MAT1A protein levels are significantly increased in female Cgl null mice with severely elevated plasma tHcy. Western blotting analysis of hepatic GNMT and MAT1A protein expression levels in male and female WT and Cgl null mice. Values shown represent the mean +/-s.d., *P<0.05, **P<0.01. Image collected and cropped by CiteAb from the following publication (https://bio.biologists.org/cgi/doi/10.1242/bio.013433) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: MAT1A Antibody [NBP1-55120]

Immunohistochemistry-Paraffin: MAT1A Antibody [NBP1-55120]

Immunohistochemistry-Paraffin: MAT1A Antibody [NBP1-55120] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: MAT1A Antibody [NBP1-55120]

Western Blot: MAT1A Antibody [NBP1-55120]

Western Blot: MAT1A Antibody [NBP1-55120] - WB analysis of MAT1A in mouse liver lysate. Image courtesy of product review submitted by Hua Jiang.
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Applications for MAT1A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1.25 ug/ml

Western Blot

1.0 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence was reported in scientific literature.

Reviewed Applications

Read 1 review rated 4 using NBP1-55120 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAT1A

MAT1A catalyzes the formation of S-adenosylmethionine from methionine and ATP. Methionine adenosyltransferase deficiency is caused by recessive and dominant mutations, the latter identified in autosomal dominant persistant hypermethioninemia.This gne encodes methionine adenosyltransferase I (alpha isoform), which catalyzes the formation of S-adenosylmethionine from methionine and ATP. Methionine adenosyltransferase deficiency is caused by recessive and dominant mutations, the latter identified in autosomal dominant persistant hypermethioninemia.

Alternate Names

AdoMet synthase 1, adoMet synthetase 1, AMS1, MAT, MATA1MAT 1, MAT-I/III, Methionine adenosyltransferase 1, methionine adenosyltransferase I, alpha, Methionine adenosyltransferase I/III, S-adenosylmethionine synthase isoform type-1, S-adenosylmethionine synthetase isoform type-1, SAMS, SAMS1EC 2.5.1.6

Entrez Gene IDs

4143 (Human)

Gene Symbol

MAT1A

UniProt

Q00266 (Human)

Additional MAT1A Products

Product Documents for MAT1A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MAT1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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