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MAT2B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82797

Novus Biologicals, part of Bio-Techne
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NBP1-82797
NBP1-82797-25ul

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Predicted:

Mouse (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLDPSIKGTFHWSGNEQMTKYEMACAIADAFN

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 20043323).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAT2B Antibody

Western Blot: MAT2B Antibody [NBP1-82797]

Western Blot: MAT2B Antibody [NBP1-82797]

Western Blot: MAT2B Antibody [NBP1-82797] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797]

Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797]

Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797] - Staining of human skin shows no positivity in keratinocytes as expected.
Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797]

Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797]

Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797] - Staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.

Applications for MAT2B Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-82797 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAT2B

MAT2B is encoded by this gene belongs to the methionine adenosyltransferase (MAT) family. MAT catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Alternate Names

beta regulatory subunit of methionine adenosyltransferase, DTDP-4-keto-6-deoxy-D-glucose 4-reductase, MAT II beta, MAT-II, MATIIbeta, methionine adenosyltransferase 2 subunit beta, Methionine adenosyltransferase II beta, methionine adenosyltransferase II, beta, MGC12237, Nbla02999, putative protein product of Nbla02999, SDR23E1, short chain dehydrogenase/reductase family 23E, member 1, TGR

Entrez Gene IDs

27430 (Human)

Gene Symbol

MAT2B

UniProt

Additional MAT2B Products

Product Documents for MAT2B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAT2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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