MATE1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36989
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
This MATE1 Antibody was developed against a peptide with sequence C-PEHPQDGAKLSRKQ corresponding to internal region (near C-Terminus) according to NP_060712.2.
Epitope
internal region (near C-Terminus)
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for MATE1 Antibody
Western Blot: MATE1 Antibody [NBP1-36989]
Western Blot: MATE1 Antibody [NBP1-36989] - Staining of Human Liver lysate (35 ug protein in RIPA buffer) with antibody at 0.1 ug/mL. Detected by chemiluminescence. Approx. 60-65kDa band observed in Human Liver lysates (calculated MW of 61.9 kDa according to NP_060712.2). This band was successfully blocked by incubation with the immunizing peptide.Immunocytochemistry/ Immunofluorescence: MATE1 Antibody [NBP1-36989]
Immunocytochemistry/Immunofluorescence: MATE1 Antibody [NBP1-36989] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). Strong expression of the protein seen in the plasma membrane of HepG2 cells.Applications for MATE1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:64000
Western Blot
0.1 - 0.3 ug/mL
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MATE1
Long Name
Multidrug and toxin extrusion protein 1
Alternate Names
hMATE-1, MATE-1, SLC47A1
Entrez Gene IDs
55244 (Human)
Gene Symbol
SLC47A1
UniProt
Additional MATE1 Products
Product Documents for MATE1 Antibody
Product Specific Notices for MATE1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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