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MATH2/NEUROD6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29865

Novus Biologicals, part of Bio-Techne
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NBP2-29865
NBP2-29865-0.025mg

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide.

Specificity

Recognizes NeuroD 6 (MATH2, NEX1), a Helix-loop-helix class of transcription factor.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Relevant Entrez Gene Numbers: NM_022728.2

Scientific Data Images for MATH2/NEUROD6 Antibody

Immunocytochemistry/ Immunofluorescence: MATH2/NEUROD6 Antibody [NBP2-29865]

Immunocytochemistry/ Immunofluorescence: MATH2/NEUROD6 Antibody [NBP2-29865]

Immunocytochemistry/Immunofluorescence: MATH2/NEUROD6 Antibody [NBP2-29865] - Confocal IF analysis of PC12 using anti NeuroD6 Rabbit pAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue).
Immunohistochemistry: MATH2/NEUROD6 Antibody [NBP2-29865]

Immunohistochemistry: MATH2/NEUROD6 Antibody [NBP2-29865]

Immunohistochemistry: MATH2/NEUROD6 Antibody [NBP2-29865] - Detects NeuroD6 in paraffin embedded rat brain tissue at 1:300 dilution.

Applications for MATH2/NEUROD6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Western Blot

1:200-1:1000
Application Notes
The rehydrated antibody solutions can be stored undiluted at 2-8C degrees for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored atTo reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 uL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 ug of precipitated antibody, the amount of buffer needed would be 50 uL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C degrees prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. 2-8C degrees .

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% saturated ammonium sulfate and PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -70C. Avoid freeze-thaw cycles.

Background: MATH2/NEUROD6

NEUROD6 is a member of the NEUROD (NEUROD1; MIM 601724) family of basic helix-loop-helix (bHLH) transcription factors (Guo et al., 2002 [PubMed 12357074]).[supplied by OMIM]

Alternate Names

Atoh2, BHLHA2, bHLHa2MATH2, Class A basic helix-loop-helix protein 2, Math-2, NeuroD6, neurogenic differentiation 6, neurogenic differentiation factor 6, NEX1M, Protein atonal homolog 2

Gene Symbol

NEUROD6

UniProt

Additional MATH2/NEUROD6 Products

Product Documents for MATH2/NEUROD6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MATH2/NEUROD6 Antibody

Relevant Entrez Gene Numbers: NM_022728.2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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