MAZ Antibody

Immunoprecipitation: MAZ Antibody [NB100-86984]

Immunoprecipitation: MAZ Antibody [NB100-86984] - Detection of human MAZ by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAZ antibody NB100-86984 (lot NB100-86984-2) used for IP at 6 ug per reaction. MAZ was also immunoprecipitated by a previous lot of this antibody (lot NB100-86984-1) and another rabbit anti-MAZ antibody. For blotting immunoprecipitated MAZ, NB100-86984 was used at 1 ug/ml. Detection: . Chemiluminescence with an exposure time of 3 minutes.

Western Blot: MAZ Antibody [NB100-86984] -

Western Blot: MAZ Antibody [NB100-86984] - MAZ is a new transcription activation target of FOXK1. (A) Analysis of the 9969 overlapping FOXK1 loci for overrepresented sequence elements identified significantly enriched FOXK1-like DNA-binding motifs, & the results showed that 12.92% of the FOXK1 genomic loci contained at least one of the motifs. (B) Potential binding sites for FOXK1 at the 5'-UTR of MAZ. (C) FOXK1 & MAZ coexpression in the TCGA GC dataset was analyzed by querying the open database ChIPBase v2.0 (r: 0.3449, p = 5.1e-14). The mRNA (D) & protein (E) levels of MAZ in MGC803 & AGS cells were attenuated by the silencing of FOXK1. The data are presented as the means ± S.D.s from three independent experiments. ** P < 0.01, *** P < 0.001, & **** P < 0.0001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32268297), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Chromatin Immunoprecipitation: MAZ Antibody [NB100-86984] -

Chromatin Immunoprecipitation: MAZ Antibody [NB100-86984] - SPIN1 controls liposarcoma cell proliferation & survival by enhancing GDNF expression in cooperation with the transcription factor MAZ(A) Venn diagram depicting the overlap of SPIN1 & MAZ locations at gene promoters in T778 cells. (B) Intensity profiles for SPIN1 & MAZ occupancy of 5,680 gene promoters around the transcription start site (TSS –/+ 2000 bp). (C) Intensity profiles of presence of SPIN1, H3K4me3, & MAZ at the GDNF gene in T778 cells determined by ChIP-sequencing. (D, E) Immunoprecipitation (IP) of endogenous SPIN1 & MAZ from T778 cell extracts with antibodies against SPIN1 or MAZ as indicated. (F, G) Quantitative RT-PCR analysis of MAZ & GDNF expression in T778 cells stably transfected with control miRNA (miCtrl) or miRNA directed against MAZ [miMAZ(1)] (F) or MAZ expression plasmid (MAZ OE) (G) Expression of miMAZ or MAZ was induced by doxycycline. Uninduced cells served as control. (F, G) Error bars represent +/– SEM, *p < 0.05, **p < 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25749382), licensed under a CC-BY license. Not internally tested by Novus Biologicals.