MBD1 Antibody (100B272.1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33221

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 100B272.1

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all MBD1 Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1; GenBank no ref|NP_056671.2|

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25798578)

Specificity

There are many isoforms of human MBD1 ranging from 503 (~ 55kD) to 655 (~ 71kD) amino acids in length. Researchers are encouraged to use the provided immunogen sequence to determine if this antibody is suitable for testing a specific isoform of interest.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MBD1 Antibody (100B272.1) - Azide and BSA Free

Knockdown Validated: MBD1 Antibody (100B272.1) - Azide and BSA Free [NBP2-33221]

Knockdown Validated: MBD1 Antibody (100B272.1) - Azide and BSA Free [NBP2-33221]

Knockdown Validated: MBD1 Antibody (100B272.1) - Azide and BSA Free [NBP2-33221] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Mouse Anti-Human MBD1 Monoclonal Antibody (Catalog # NBP2-33221) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: MBD1 Antibody (100B272.1)Azide and BSA Free [NBP2-33221]

Western Blot: MBD1 Antibody (100B272.1)Azide and BSA Free [NBP2-33221]

Western Blot: MBD1 Antibody (100B272.1) - Azide and BSA Free [NBP2-33221] - Analysis of MBD1 in HeLa lysate using MBD1 antibody at 2 ug/ml (lane 1) and 1 ug/ml (lane 2).
Western Blot: MBD1 Antibody (100B272.1)Azide and BSA Free [NBP2-33221]

Western Blot: MBD1 Antibody (100B272.1)Azide and BSA Free [NBP2-33221]

Western Blot: MBD1 Antibody (100B272.1) - Azide and BSA Free [NBP2-33221] - Analysis of Jurkat cell lysate using anti-MBD1 antibody. Image from verified customer review.

Applications for MBD1 Antibody (100B272.1) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10-1:500. Use reported in scientific literature (PMID 25798578)

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunoprecipitation

1:10-1:500. Use reported in scientific literature (PMID 15327775)

Western Blot

2-4 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBD1

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Alternate Names

CXXC-type zinc finger protein 3, methyl-CpG binding domain protein 1, methyl-CpG binding domain protein 1 isoform PCM1, methyl-CpG-binding domain protein 1, Methyl-CpG-binding protein MBD1, PCM1CXXC3RFT, Protein containing methyl-CpG-binding domain 1, the regulator of fibroblast growth factor 2 (FGF-2) transcription

Entrez Gene IDs

4152 (Human)

Gene Symbol

MBD1

UniProt

Q9UIS9 (Human)

Additional MBD1 Products

Product Documents for MBD1 Antibody (100B272.1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MBD1 Antibody (100B272.1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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