MBD1 Antibody (10B10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26147

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26147-100ul
NBP3-26147-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10B10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MBD1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human MBD1 [UniProt Q9UIS9]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MBD1 Antibody (10B10)

MBD1 Antibody (10B10)

Immunocytochemistry/Immunofluorescence: MBD1 Antibody (10B10) [NBP3-26147] -

Immunocytochemistry/Immunofluorescence: MBD1 Antibody (10B10) [NBP3-26147] - Staining of MCF-7 cell with MBD1 Antibody (10B10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 498-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for MBD1 Antibody (10B10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: MBD1

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

Alternate Names

CXXC-type zinc finger protein 3, methyl-CpG binding domain protein 1, methyl-CpG binding domain protein 1 isoform PCM1, methyl-CpG-binding domain protein 1, Methyl-CpG-binding protein MBD1, PCM1CXXC3RFT, Protein containing methyl-CpG-binding domain 1, the regulator of fibroblast growth factor 2 (FGF-2) transcription

Gene Symbol

MBD1

Additional MBD1 Products

Product Documents for MBD1 Antibody (10B10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MBD1 Antibody (10B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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