MBD1 Antibody

Western Blot: MBD1 Antibody [NBP3-18674]

Western Blot: MBD1 Antibody [NBP3-18674] - Figure 2. Western blot analysis using the antibody directed against MBD1 HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the antibody against MBD1 diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.

ELISA: MBD1 Antibody [NBP3-18674]

ELISA: MBD1 Antibody [NBP3-18674] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MBD1 in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000.