MBD5 Antibody (4A12-1B6)
Novus Biologicals, part of Bio-Techne | Catalog # H00055777-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4A12-1B6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MBD5 (AAH14534.1, 1 a.a. ~ 229 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGMVLLEKSTQRY
Reactivity Notes
Human. Other species not tested.
Specificity
MBD5 - methyl-CpG binding domain protein 5
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MBD5 Antibody (4A12-1B6)
![Western Blot: MBD5 Antibody (4A12-1B6) [H00055777-M01]](https://resources.bio-techne.com/images/products/MBD5-Antibody-4A12-1B6-Western-Blot-H00055777-M01-img0005.jpg "Western Blot: MBD5 Antibody (4A12-1B6) [H00055777-M01]")
Western Blot: MBD5 Antibody (4A12-1B6) [H00055777-M01]
Western Blot: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of MBD5 expression in transfected 293T cell line by MBD5 monoclonal antibody (M01), clone 4A12-1B6.Lane 1: MBD5 transfected lysate(24.3 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: MBD5 Antibody (4A12-1B6) [H00055777-M01]](https://resources.bio-techne.com/images/products/MBD5-Antibody-4A12-1B6-Immunocytochemistry-Immunofluorescence-H00055777-M01-img0003.jpg "Immunocytochemistry/ Immunofluorescence: MBD5 Antibody (4A12-1B6) [H00055777-M01]")
Immunocytochemistry/ Immunofluorescence: MBD5 Antibody (4A12-1B6) [H00055777-M01]
Immunocytochemistry/Immunofluorescence: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of monoclonal antibody to MBD5 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Detection limit for recombinant GST tagged MBD5 is 0.03 ng/ml as a capture antibody.
Applications for MBD5 Antibody (4A12-1B6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Western Blot
1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MBD5
Alternate Names
KIAA1461, methyl-CpG binding domain protein 5, methyl-CpG-binding domain protein 5, methyl-CpG-binding protein MBD5, MRD1
Entrez Gene IDs
55777 (Human)
Gene Symbol
MBD5
Additional MBD5 Products
Product Documents for MBD5 Antibody (4A12-1B6)
Product Specific Notices for MBD5 Antibody (4A12-1B6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...