MCAF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84610

Novus Biologicals, part of Bio-Techne
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NBP1-84610
NBP1-84610-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MCAF1 Antibodies »

Product Summary for MCAF1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELTCFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCAF1 Antibody

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Analysis in human testis and skeletal muscle tissues. Corresponding MCAF1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: MCAF1 Antibody [NBP1-84610]

Immunocytochemistry/ Immunofluorescence: MCAF1 Antibody [NBP1-84610]

Immunocytochemistry/Immunofluorescence: MCAF1 Antibody [NBP1-84610] - Staining of human cell line A-431 shows positivity in nuclei but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
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Applications for MCAF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000-1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-84610 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCAF1

MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action (1).

Alternate Names

activating transcription factor 7 interacting protein, activating transcription factor 7-interacting protein 1, ATF7-interacting protein, ATFa-associated modulator, ATF-interacting protein, ATF-IP, FLJ10139, FLJ10688, hAM, MBD1-containing chromatin associated factor, MCAF1AM, MCAFMBD1-containing chromatin-associated factor 1, MMCP 6, MMCP6, MMCP-6, p621

Gene Symbol

ATF7IP

Additional MCAF1 Products

Product Documents for MCAF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MCAF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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