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MCAF1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03759

Novus Biologicals, part of Bio-Techne
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NBP3-03759-100ul
NBP3-03759-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for MCAF1 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 525-725 of human MCAF1 (NP_060649.3). NEKDNKPEEEEQVIHEDDERPSEKNEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVIQWLLEEKLCALQCAVFDKTLAELKTRVEKIECNKRHKTVLTELQAKIARLTKRFEAAKEDLKKRHEHPPNPPVSPGKTVNDVNSNNNMSYRNAGTVRQMLESKRNVSESAPPSFQTPVNTVSSTNL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCAF1 Antibody - BSA Free

Western Blot: MCAF1 AntibodyBSA Free [NBP3-03759]

Western Blot: MCAF1 AntibodyBSA Free [NBP3-03759]

Western Blot: MCAF1 Antibody [NBP3-03759] - Western blot analysis of extracts of various cell lines, using MCAF1 antibody (NBP3-03759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Applications for MCAF1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCAF1

MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action (1).

Alternate Names

activating transcription factor 7 interacting protein, activating transcription factor 7-interacting protein 1, ATF7-interacting protein, ATFa-associated modulator, ATF-interacting protein, ATF-IP, FLJ10139, FLJ10688, hAM, MBD1-containing chromatin associated factor, MCAF1AM, MCAFMBD1-containing chromatin-associated factor 1, MMCP 6, MMCP6, MMCP-6, p621

Gene Symbol

ATF7IP

Additional MCAF1 Products

Product Documents for MCAF1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MCAF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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