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MCCC2 Antibody (2B3)

Novus Biologicals, part of Bio-Techne | Catalog # H00064087-M05

Novus Biologicals, part of Bio-Techne
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H00064087-M05

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2B3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MCCC2 (NP_071415, 456 a.a. ~ 563 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AYSPRFLYIWPNARISVMGGEQAANVLATITKDQRAREGKQFSSADEAALKEPIIKKFEEEGNPYYSSARVWDDGIIDPADTRLVLGLSFSAALNAPIEKTDFGIFRM

Specificity

MCCC2 - methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (2B3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MCCC2 Antibody (2B3)

Western Blot: MCCC2 Antibody (2B3) [H00064087-M05]

Western Blot: MCCC2 Antibody (2B3) [H00064087-M05]

Western Blot: MCCC2 Antibody (2B3) [H00064087-M05] - Analysis of MCCC2 expression in transfected 293T cell line by MCCC2 monoclonal antibody (M05), clone 2B3. Lane 1: MCCC2 transfected lysate (Predicted MW: 61.3 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: MCCC2 Antibody (2B3) [H00064087-M05]

Immunohistochemistry-Paraffin: MCCC2 Antibody (2B3) [H00064087-M05]

Immunohistochemistry-Paraffin: MCCC2 Antibody (2B3) [H00064087-M05] - Analysis of monoclonal antibody to MCCC2 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml
Sandwich ELISA: MCCC2 Antibody (2B3) [H00064087-M05] - Detection limit for recombinant GST tagged MCCC2 is 0.1 ng/ml as a capture antibody.

Applications for MCCC2 Antibody (2B3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MCCC2

This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. [provided by RefSeq]

Alternate Names

3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta, MCCase subunit beta, MCCBEC 6.4.1.4, methylcrotonoyl-CoA carboxylase 2 (beta), methylcrotonoyl-Coenzyme A carboxylase 2 (beta), mitochondrial, non-biotin containing subunit of 3-methylcrotonyl-CoA carboxylase

Entrez Gene IDs

64087 (Human)

Gene Symbol

MCCC2

Additional MCCC2 Products

Product Documents for MCCC2 Antibody (2B3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MCCC2 Antibody (2B3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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