MCM2 Antibody (0Q10S9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16066

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16066-100ul
NBP3-16066-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0Q10S9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MCM2 Antibodies »

Product Summary for MCM2 Antibody (0Q10S9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MCM2 (P49736). MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM2 Antibody (0Q10S9)

Western Blot: MCM2 Antibody (0Q10S9) [NBP3-16066]

Western Blot: MCM2 Antibody (0Q10S9) [NBP3-16066]

Western Blot: MCM2 Antibody (0Q10S9) [NBP3-16066] - Western blot analysis of extracts of various cell lines, using MCM2 antibody (NBP3-16066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.3s.
Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (0Q10S9) [NBP3-16066]

Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (0Q10S9) [NBP3-16066]

Immunocytochemistry/Immunofluorescence: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunofluorescence analysis of U2OS cells using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MCM2 Antibody (0Q10S9) [NBP3-16066]

Immunohistochemistry-Paraffin: MCM2 Antibody (0Q10S9) [NBP3-16066]

Immunohistochemistry-Paraffin: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry of paraffin-embedded rat spleen using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for MCM2 Antibody (0Q10S9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCM2

MCM2 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Long Name

Minichromosome Maintenance Protein 2

Alternate Names

BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin

Gene Symbol

MCM2

Additional MCM2 Products

Product Documents for MCM2 Antibody (0Q10S9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MCM2 Antibody (0Q10S9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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