MCM4 Antibody (5N0X3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16791

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16791-100ul
NBP3-16791-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5N0X3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MCM4 Antibodies »

Product Summary for MCM4 Antibody (5N0X3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 764-863 of human MCM4 (P33991). LHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKTVRLL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM4 Antibody (5N0X3)

Western Blot: MCM4 Antibody (5N0X3) [NBP3-16791]

Western Blot: MCM4 Antibody (5N0X3) [NBP3-16791]

Western Blot: MCM4 Antibody (5N0X3) [NBP3-16791] - Western blot analysis of extracts of various cell lines, using MCM4 Rabbit mAb (NBP3-16791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for MCM4 Antibody (5N0X3)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCM4

MCM4 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Alternate Names

CDC21, CDC21 homolog, CDC54, DNA replication licensing factor MCM4, EC 3.6.4.12, hCdc21, homolog of S. pombe cell devision cycle 21, MCM4 minichromosome maintenance deficient 4, MCM4 minichromosome maintenance deficient 4 (S. cerevisiae), MGC33310, minichromosome maintenance complex component 4, minichromosome maintenance deficient 4, P1-Cdc21

Gene Symbol

MCM4

Additional MCM4 Products

Product Documents for MCM4 Antibody (5N0X3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MCM4 Antibody (5N0X3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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