MCM5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85722

Novus Biologicals, part of Bio-Techne
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NBP1-85722
NBP1-85722-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MCM5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM5 Antibody

Western Blot: MCM5 Antibody [NBP1-85722]

Western Blot: MCM5 Antibody [NBP1-85722]

Western Blot: MCM5 Antibody [NBP1-85722] - Analysis in control (vector only transfected HEK293T lysate) and MCM5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: MCM5 Antibody [NBP1-85722]

Immunohistochemistry-Paraffin: MCM5 Antibody [NBP1-85722]

Immunohistochemistry-Paraffin: MCM5 Antibody [NBP1-85722] - Staining in human bone marrow and pancreas tissues using anti-MCM5 antibody. Corresponding MCM5 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MCM5 Antibody [NBP1-85722]

Immunohistochemistry-Paraffin: MCM5 Antibody [NBP1-85722]

Immunohistochemistry-Paraffin: MCM5 Antibody [NBP1-85722] - Staining of human bone marrow shows high expression.
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Applications for MCM5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 -1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM5

MCM5 (Mini Chromosome Maintenance protein-5) is involved in the control of DNA replication. MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increase as the cell enters G1/S phase of the cell cycle. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.

Alternate Names

CDC46 homolog, CDC46DNA replication licensing factor MCM5, EC 3.6.4.12, MCM5 minichromosome maintenance deficient 5, cell division cycle 46, MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S.cerevisiae), MGC5315, minichromosome maintenance complex component 5, minichromosome maintenance deficient (S. cerevisiae) 5 (cell division cycle 46), minichromosome maintenance deficient 5 (cell division cycle 46), P1-CDC46

Gene Symbol

MCM5

Additional MCM5 Products

Product Documents for MCM5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MCM5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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