MCM7 Antibody (MCM7/1467)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59608

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # MCM7/1467

Concentration

0.2 mg/ml

View all MCM7 Antibodies »

Product Summary for MCM7 Antibody (MCM7/1467)

Immunogen

Recombinant human MCM7 protein fragment (aa195-319) (exact sequence is proprietary) (Uniprot: P33993)

Localization

Nuclear

Specificity

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Marker

Proliferation Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Human Chromosome Location:7q21.3-q22.1


200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-59609)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MCM7 Antibody (MCM7/1467)

Western Blot: MCM7 Antibody (MCM7/1467) [NBP2-59608]

Western Blot: MCM7 Antibody (MCM7/1467) [NBP2-59608]

Western Blot: MCM7 Antibody (MCM7/1467) [NBP2-59608] - Western Blot Analysis of MCF-7, HEK-293, A549 lysate using MCM7 Antibody (MCM7/1467).
Immunohistochemistry-Paraffin: MCM7 Antibody (MCM7/1467) [NBP2-59608]

Immunohistochemistry-Paraffin: MCM7 Antibody (MCM7/1467) [NBP2-59608]

Immunohistochemistry-Paraffin: MCM7 Antibody (MCM7/1467) [NBP2-59608] - Formalin-fixed, paraffin-embedded human Tonsil stained withMCM7 Mouse Monoclonal Antibody (MCM7/1467).
Protein Array: MCM7 Antibody (MCM7/1467) [NBP2-59608]

Protein Array: MCM7 Antibody (MCM7/1467) [NBP2-59608]

Protein Array: MCM7 Antibody (MCM7/1467) [NBP2-59608] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MCM7 Antibody (MCM7/1467) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for MCM7 Antibody (MCM7/1467)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Protein Array

1:100-1:2000
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MCM7

The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3).

Long Name

Minichromosome Maintenance Protein 7

Alternate Names

CDC47, MCM2, P1CDC47, P85MCM, PNAS146, PPP1R104, Protein Phosphatase 1

Gene Symbol

MCM7

UniProt

P33993

Additional MCM7 Products

Product Documents for MCM7 Antibody (MCM7/1467)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MCM7 Antibody (MCM7/1467)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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