MCM7 Antibody (SPM379)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53219
![Western Blot: MCM7 Antibody (SPM379) [NBP2-53219]](https://resources.bio-techne.com/images/products/MCM7-Antibody-SPM379-Western-Blot-NBP2-53219-img0002.jpg "Western Blot: MCM7 Antibody (SPM379) [NBP2-53219]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM379
Concentration
0.2 mg/ml
Product Summary for MCM7 Antibody (SPM379)
Immunogen
Recombinant human MCM7 protein (Uniprot: P33993)
Localization
Nuclear
Marker
Proliferation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54460)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for MCM7 Antibody (SPM379)
Western Blot: MCM7 Antibody (SPM379) [NBP2-53219]
Western Blot: MCM7 Antibody (SPM379) [NBP2-53219] - Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Antibody (SPM379).![Immunohistochemistry-Paraffin: MCM7 Antibody (SPM379) [NBP2-53219]](https://resources.bio-techne.com/images/products/MCM7-Antibody-SPM379-Immunohistochemistry-Paraffin-NBP2-53219-img0001.jpg "Immunohistochemistry-Paraffin: MCM7 Antibody (SPM379) [NBP2-53219]")
Immunohistochemistry-Paraffin: MCM7 Antibody (SPM379) [NBP2-53219]
Immunohistochemistry-Paraffin: MCM7 Antibody (SPM379) [NBP2-53219] - Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Monoclonal Antibody (SPM379).Applications for MCM7 Antibody (SPM379)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: MCM7
Long Name
Minichromosome Maintenance Protein 7
Alternate Names
CDC47, MCM2, P1CDC47, P85MCM, PNAS146, PPP1R104, Protein Phosphatase 1
Gene Symbol
MCM7
UniProt
Additional MCM7 Products
Product Documents for MCM7 Antibody (SPM379)
Product Specific Notices for MCM7 Antibody (SPM379)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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