MCM8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-58129

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-58129
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all MCM8 Antibodies »

Product Summary for MCM8 Antibody

Immunogen

Synthetic peptides corresponding to MCM8(minichromosome maintenance complex component 8) The peptide sequence was selected from the C terminal of human MCM8. Peptide sequence IRLTEARARLELREEATKEDAEDIVEIMKYSMLGTYSDEFGNLDFERSQH. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for MCM8 Antibody

Western Blot: MCM8 Antibody [NBP1-58129]

Western Blot: MCM8 Antibody [NBP1-58129]

Western Blot: MCM8 Antibody [NBP1-58129] - 721_B cell lysate, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry-Paraffin: MCM8 Antibody [NBP1-58129]

Immunohistochemistry-Paraffin: MCM8 Antibody [NBP1-58129]

Immunohistochemistry-Paraffin: MCM8 Antibody [NBP1-58129] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X magnification.

Applications for MCM8 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

4-8 ug/ml

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM8

MCM8 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. This protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. This protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Alternate Names

C20orf154, chromosome 20 open reading frame 154, dJ967N21.5, DNA replication licensing factor MCM8, MCM8 minichromosome maintenance deficient 8, MGC119522, MGC119523, MGC12866, MGC4816, Minichromosome maintenance 8, minichromosome maintenance complex component 8, REC, REC homolog

Entrez Gene IDs

84515 (Human)

Gene Symbol

MCM8

UniProt

Q9UJA3 (Human)

Additional MCM8 Products

Product Documents for MCM8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MCM8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.