MCPIP1/ZC3H12A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37871
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: CTYGIKCRFFHPERPSCPQRSVADELRANALLSPPRAPSKDKNGRRPSPSSQSSSLLTESEQCSLDGKKLGAQASPGSRQEGLTQTYAPSGRSLAP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MCPIP1/ZC3H12A Antibody
Western Blot: MCPIP1/ZC3H12A Antibody [NBP2-37871]
Western Blot: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Analysis in control (vector only transfected HEK293T lysate) and ZC3H12A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: MCPIP1/ZC3H12A Antibody [NBP2-37871]
Immunocytochemistry/Immunofluorescence: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human cell line U-251 MG shows localization to cytoplasmic bodies. Antibody staining is shown in green.Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871]
Immunohistochemistry-Paraffin: MCPIP1/ZC3H12A Antibody [NBP2-37871] - Staining of human skeletal muscle shows no positivity in myocytes as expected.Applications for MCPIP1/ZC3H12A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MCPIP1
References
1. Uehata, T., & Takeuchi, O. (2017). Regnase-1 Is an Endoribonuclease Essential for the Maintenance of Immune Homeostasis. Journal of interferon & cytokine research: the official journal of the International Society for Interferon and Cytokine Research, 37(5), 220-229. https://doi.org/10.1089/jir.2017.0001
2. Miekus, K., Kotlinowski, J., Lichawska-Cieslar, A., Rys, J., & Jura, J. (2019). Activity of MCPIP1 RNase in tumor associated processes. Journal of experimental & clinical cancer research : CR, 38(1), 421. https://doi.org/10.1186/s13046-019-1430-6
3. Jura, J., Skalniak, L., & Koj, A. (2012). Monocyte chemotactic protein-1-induced protein-1 (MCPIP1) is a novel multifunctional modulator of inflammatory reactions. Biochimica et biophysica acta, 1823(10), 1905-1913. https://doi.org/10.1016/j.bbamcr.2012.06.029
4. Xu, J., Fu, S., Peng, W., & Rao, Z. (2012). MCP-1-induced protein-1, an immune regulator. Protein & cell, 3(12), 903-910. https://doi.org/10.1007/s13238-012-2075-9
5. Musson, R., Szukala, W., & Jura, J. (2020). MCPIP1 RNase and Its Multifaceted Role. International journal of molecular sciences, 21(19), 7183. https://doi.org/10.3390/ijms21197183
Long Name
MCP-1-induced Protein 1
Alternate Names
ZC3H12A
Gene Symbol
ZC3H12A
UniProt
Additional MCPIP1 Products
Product Documents for MCPIP1/ZC3H12A Antibody
Product Specific Notices for MCPIP1/ZC3H12A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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