MD-2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77201

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all MD2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 13 amino acids near the center of human MD-2. The immunogen is located within the last 50 amino acids of MD-2. Amino Acid Sequence: CSFKGIKFSKGKYK

Specificity

Human MD-2 has 2 isoforms, including isoform 1 (160aa, 18kD) and isoform 2 (130aa, 15kD). Mouse MD-2 has one isoform (160aa, 18kD) and Rat MD-2 also has one isoform (160aa, 18kD). NBP1-77201 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MD-2 Antibody - BSA Free

Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Rat Tissue Lysates. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry-Paraffin: MD-2 Antibody - BSA Free [NBP1-77201]

Immunohistochemistry-Paraffin: MD-2 Antibody - BSA Free [NBP1-77201]

Immunohistochemistry-Paraffin: MD-2 Antibody [NBP1-77201] - Analysis of MD-2 in human spleen tissue with MD-2 antibody at 2.5 ug/ml.
Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MD-2 recombinant protein per lane. Antibodies: MD-2, NBP1-77201 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL and Lane 3: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Applications for MD-2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/mL

Immunohistochemistry

2-2.5 ug/mL

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

0.5-1 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MD2

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Long Name

Myeloid Differentiation Protein 2/Lymphocyte Antigen 96

Alternate Names

ESOP-1, LY96, MD-2

Gene Symbol

LY96

UniProt

NP_056179 (Human)

Additional MD2 Products

Product Documents for MD-2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MD-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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