MDA5 Antibody (PSH03-56)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32581
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH03-56
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human MDA5 aa 250-1,025 / 1,025. (Uniprot: Q9BYX4)
Localization
Cytoplasm, Nucleus, Mitochondrion.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MDA5 Antibody (PSH03-56)
Western Blot: MDA5 Antibody (PSH03-56) [NBP3-32581]
Western Blot: MDA5 Antibody (PSH03-56) [NBP3-32581] - Western blot analysis of MDA5 on different lysates with Rabbit anti-MDA5 antibody (NBP3-32581) at 1/2,000 dilution.Lane 1: THP-1 cell lysate (20 ug/Lane)
Lane 2: HepG2 cell lysate (20 ug/Lane)
Lane 3: Jurkat cell lysate (20 ug/Lane)
Lane 4: Daudi cell lysate (20 ug/Lane)
Lane 5: Raji cell lysate (20 ug/Lane)
Lane 6: MCF7 cell lysate (20 ug/Lane)
Lane 7: HeLa cell lysate (20 ug/Lane)
Lane 8: SH-SY5Y cell lysate (20 ug/Lane)
Lane 9: A549 cell lysate (20 ug/Lane)
Lane 10: A431 cell lysate (20 ug/Lane)
Lane 11: Mouse pancreas tissue lysate (40 ug/Lane)
Lane 12: Rat kidney tissue lysate (40 ug/Lane)
Lysates/proteins at 10 ug/Lane.
Predicted band size: 117 kDa
Observed band size: 140/100 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32581) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for MDA5 Antibody (PSH03-56)
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IFIH1/MDA5
Long Name
Melanoma Differentiation-Associated Protein 5
Alternate Names
AGS7, Helicard, Hlcd, IDDM19, IFIH1, IMD95, MDA-5, RH116, SGMRT1
Gene Symbol
IFIH1
Additional IFIH1/MDA5 Products
Product Documents for MDA5 Antibody (PSH03-56)
Product Specific Notices for MDA5 Antibody (PSH03-56)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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