MDFI Antibody (3B4)

Novus Biologicals, part of Bio-Techne | Catalog # H00004188-M07

Novus Biologicals, part of Bio-Techne
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H00004188-M07
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MDFI Antibodies »

Product Specifications

Immunogen

MDFI (NP_005577, 33 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PGLEVVTGSTHPAEAAPEEGSLEEAATPMPQGNGPGIPQGLDSTDLDVPTEAVTCQPQGNPLGCTPLLPNDSGHPSEL

Specificity

MDFI - MyoD family inhibitor (3B4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MDFI Antibody (3B4)

Western Blot: MDFI Antibody (3B4) [H00004188-M07]

Western Blot: MDFI Antibody (3B4) [H00004188-M07]

Western Blot: MDFI Antibody (3B4) [H00004188-M07] - Analysis of MDFI expression in transfected 293T cell line by MDFI monoclonal antibody (M07), clone 3B4. Lane 1: MDFI transfected lysatE (25.029 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: MDFI Antibody (3B4) [H00004188-M07]

Immunocytochemistry/ Immunofluorescence: MDFI Antibody (3B4) [H00004188-M07]

Immunocytochemistry/Immunofluorescence: MDFI Antibody (3B4) [H00004188-M07] - Analysis of monoclonal antibody to MDFI on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: MDFI Antibody (3B4) [H00004188-M07]

Western Blot: MDFI Antibody (3B4) [H00004188-M07]

Western Blot: MDFI Antibody (3B4) [H00004188-M07] - detection against Immunogen (34.69 KDa) .

Applications for MDFI Antibody (3B4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MDFI

This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq]

Alternate Names

I-MF, I-mfa, inhibitor of MyoD family a, MyoD family inhibitor, Myogenic repressor I-mf

Entrez Gene IDs

4188 (Human)

Gene Symbol

MDFI

UniProt

NP_005577 (Human)

Additional MDFI Products

Product Documents for MDFI Antibody (3B4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MDFI Antibody (3B4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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