MDFI Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45224

Novus Biologicals, part of Bio-Techne
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NBP1-45224
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MDFI Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SKSTTSQIPLQAQED corresponding to internal region according to NP_005577.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MDFI Antibody

Immunohistochemistry-Paraffin: MDFI Antibody [NBP1-45224]

Immunohistochemistry-Paraffin: MDFI Antibody [NBP1-45224]

Immunohistochemistry-Paraffin: MDFI Antibody [NBP1-45224] - (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for MDFI Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx. 70kDa band in lysates of cell line HepG2 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25kDa according to NP_005577.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Lung (Bronchus).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MDFI

MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq]

Alternate Names

I-MF, I-mfa, inhibitor of MyoD family a, MyoD family inhibitor, Myogenic repressor I-mf

Entrez Gene IDs

4188 (Human)

Gene Symbol

MDFI

UniProt

NP_005577.1 (Human)

Additional MDFI Products

Product Documents for MDFI Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MDFI Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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