MDFI Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45224
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-SKSTTSQIPLQAQED corresponding to internal region according to NP_005577.1.
Epitope
internal region
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for MDFI Antibody
![Immunohistochemistry-Paraffin: MDFI Antibody [NBP1-45224]](https://resources.bio-techne.com/images/products/MDFI-Antibody-Immunohistochemistry-Paraffin-NBP1-45224-img0001.jpg "Immunohistochemistry-Paraffin: MDFI Antibody [NBP1-45224]")
Immunohistochemistry-Paraffin: MDFI Antibody [NBP1-45224]
Immunohistochemistry-Paraffin: MDFI Antibody [NBP1-45224] - (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for MDFI Antibody
Application
Recommended Usage
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Peptide ELISA
Detection limit 1:16000
Western Blot
0.3 ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx. 70kDa band in lysates of cell line HepG2 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25kDa according to NP_005577.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Lung (Bronchus).
IHC: Paraffin embedded Human Lung (Bronchus).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MDFI
Alternate Names
I-MF, I-mfa, inhibitor of MyoD family a, MyoD family inhibitor, Myogenic repressor I-mf
Entrez Gene IDs
4188 (Human)
Gene Symbol
MDFI
UniProt
Additional MDFI Products
Product Documents for MDFI Antibody
Product Specific Notices for MDFI Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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