MDH1 Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2019
Conjugate
Catalog #
Key Product Details
Species Reactivity
Porcine
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
HRP
Antibody Source
Polyclonal Sheep IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
MDH1 [Pig Heart] (Uniprot: P11708)
Reactivity Notes
Cross reactivity against MDH1 from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]
Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]
Scientific Data Images for MDH1 Antibody [HRP]
Western Blot: MDH1 Antibody [HRP] [NB100-2019] - Western Blot of Sheep MDH1 antibody [HRP]. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: MDH1 [0.05ug]. Primary Antibody: Anti-MDH1 HRP at 1:1000 for 1 hr at RT. Secondary Antibody: none. Blocking Buffer for 30mins at RT. Predicted MW: 30-35kDa. Exposure: 1sec.
Applications for MDH1 Antibody [HRP]
Application
Recommended Usage
ELISA
1:10000-1:50000
Immunohistochemistry
1:500-1:2500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:100
Western Blot
1:1000-1:5000
Application Notes
This product has been tested by western blot and is useful in ELISA and immunohistochemistry. Optimal titers should be obtained by researchers.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: MDH1
Long Name
malate dehydrogenase 1
Alternate Names
DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2
Gene Symbol
MDH1
UniProt
Additional MDH1 Products
Product Specific Notices for MDH1 Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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