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MDH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89515

Novus Biologicals, part of Bio-Techne
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NBP1-89515
NBP1-89515-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (96%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDH1 Antibody

Western Blot: MDH1 Antibody [NBP1-89515]

Western Blot: MDH1 Antibody [NBP1-89515]

Western Blot: MDH1 Antibody [NBP1-89515] - Analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: MDH1 Antibody [NBP1-89515]

Immunocytochemistry/ Immunofluorescence: MDH1 Antibody [NBP1-89515]

Immunocytochemistry/Immunofluorescence: MDH1 Antibody [NBP1-89515] - Staining of human cell line U-251 MG shows localization to cytosol & centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MDH1 Antibody [NBP1-89515]

Immunohistochemistry-Paraffin: MDH1 Antibody [NBP1-89515]

Immunohistochemistry-Paraffin: MDH1 Antibody [NBP1-89515] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Applications for MDH1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDH1

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. (provided by RefSeq)

Long Name

malate dehydrogenase 1

Alternate Names

DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2

Gene Symbol

MDH1

Additional MDH1 Products

Product Documents for MDH1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MDH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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